Sanger sequencing was used in the first generation of DNA sequencers. Despite miniaturisation and a certain amount of automation, it was st...

You've taken your sample, extracted the DNA, amplified the 16S rDNA in a PCR machine, run a DGGE gel and you end up with something li...

Denaturing and temperature gradient gel electrophoresis (DGGE and TGGE) are methods of generating fingerprints of microbial communities. DG...

This post is carrying on from the  previous one , examining a model of clone libraries to work out which analysis we can rely on to give us...

Clone libraries are usually used to try and define which bacteria are found in different places. However, it might be tempting to infer abu...

To summarise the process so far, we've: 1. Taken a sample and either stored it or immediately extracted the DNA from the bacteria pr...